For optimal results recommended primer concentrations should.
Sybr green pcr master mix.
Find the best master mix by application.
Power sybr green pcr master mix contains all of the components excluding the template and primers for superior sybr green reagent based real time pcr.
Specific minimize primer dimer and non specific amplication reproducible and sensitive consistent amplification across a wide dynamic range.
Powerup sybr green master mix is a pre formulated optimized universal 2x master mix for real time pcr workflows.
Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region.
Coupled with user supplied primer sets and template powerup sybr green master mix is designed to amplifiy targets for accurate gene expression analysis.
Sybr select master mix is a superior and cost effective solution for your real time pcr applications.
Applied biosystems amplitaq gold dna polymerase ld is a hot start pcr enzyme provided in an inactive state allowing for room temperature set up and minimizing non specific amplification.
Sybr green pcr master mix in a 1 ml vial.
As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step.
2 it is also compatible with the bio rad iq 5 and cfx systems roche lightcycler 480 and agilent mx3005p systems.
It contains faststart taq dna polymerase and dna double strand specific sybr green i dye for product detection and characterization.
Sufficient reagents provided for 40 reactions based on a 50 µl reaction volume.
Powerup sybr green master mix can be used in either standard or fast cycling mode and is compatible with all applied biosystems real time pcr instruments.
Sybr green master mix advantages.
Guaranteed minimum shelf life is 60 days exact expiry date printed on product and cofa.
